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dc.contributor.authorBayram, Nazende Nur
dc.contributor.authorTopuzogullari, Murat
dc.contributor.authorIsoglu, Ismail Alper
dc.contributor.authorIsoglu, Sevil Dincer
dc.date.accessioned2022-02-15T13:02:59Z
dc.date.available2022-02-15T13:02:59Z
dc.date.issued2021en_US
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.urihttps //doi.org/10.1007/s00289-021-03964-8
dc.identifier.urihttps://hdl.handle.net/20.500.12573/1140
dc.descriptionThis study was supported by the Scientific Research Fund of the Abdullah Gul University (Project Number: FOA-2017-81).en_US
dc.description.abstractTo achieve high stability and biocompatibility in physiological environment, oligoethyleneglycol methacrylate (OEGMA) and 4-vinylpyridine (4VP)-based amphiphilic block copolymers were prepared as micellar carriers to deliver doxorubicin into tumor cells. First, macroinitiator of OEGMA was synthesized by RAFT polymerization at [M](0)/[CTA](0)/[I](0) ratio of 100/1/0.2 in dimethylformamide (DMF) at 70 degrees C, in the presence of 4,4'-azobis(4-cyanovaleric acid) (ACVA) as initiator and 4-cyano-4-(thiobenzoylthio)pentanoic acid (CTA) as chain transfer agent, respectively. It was followed by copolymerization with 4-VP at similar conditions. The formation of RAFT-mediated polymers was approved by FTIR, H-1-NMR and GPC. For the preparation of drug-loaded micelles, a dialysis method was applied and hydrophobic doxorubicin, as a model drug, was entrapped into the micelles. Size distributions and morphologies of drug-loaded micelles were investigated by light scattering and scanning electron microscopy, respectively. Critical micelle concentration was estimated as 0.0019 mg/mL by measuring light scattering intensity in different polymer concentrations. Also, drug loading and entrapment efficiencies were calculated as 4.41% and 17.65% by measuring the DOX amount in the micelles, spectrophotometrically. At last, the drug-loaded micelles were applied to SKBR-3 breast cancer cell lines and revealed up to %40 cell inhibition at 48 and 72 h. As a result, these nanosized and biocompatible micelles can be used for the delivery of hydrophobic drugs, and they can also be modified for further targeting and imaging applications toward specific cancer cells.en_US
dc.description.sponsorshipScientific Research Fund of the Abdullah Gul University FOA-2017-81en_US
dc.language.isoengen_US
dc.publisherSPRINGERONE NEW YORK PLAZA, SUITE 4600 , NEW YORK, NY 10004, UNITED STATESen_US
dc.relation.isversionof10.1007/s00289-021-03964-8en_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast canceren_US
dc.subjectpH-responsiveen_US
dc.subjectMicelle nanocarrieren_US
dc.subjectRAFTen_US
dc.titleRAFT-synthesized POEGMA-b-P4VP block copolymers: preparation of nanosized micelles for anticancer drug releaseen_US
dc.typearticleen_US
dc.contributor.departmentAGÜ, Yaşam ve Doğa Bilimleri Fakültesi, Biyomühendislik Bölümüen_US
dc.contributor.authorID0000-0002-8697-1654en_US
dc.contributor.institutionauthorBayram, Nazende Nur
dc.contributor.institutionauthorIsoglu, Ismail Alper
dc.contributor.institutionauthorIsoglu, Sevil Dincer
dc.relation.journalPOLYMER BULLETINen_US
dc.relation.publicationcategoryMakale - Uluslararası - Editör Denetimli Dergien_US


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