Magnetic Separation of Micro Beads and Cells on a Paper-Based Lateral Flow System
Özet
Paper based lateral flow systems are widely used biosensor platforms to detect biomolecules in a
liquid sample. Proteins, bacteria, oligonucleotides, and nanoparticles were investigated in the
literature. In this work we designed a magnetic platform including dual magnets and tested the
flow of micron size immunomagnetic particles alone and when loaded with cells on two different
types of papers. The prewetting conditions of the paper and the applied external magnetic field
are the two dominant factors affecting the particle and cell transport in paper. The images recorded
with a cell phone, or with a bright field optical microscope were analyzed to measure the flow of
particles and cells. The effect of prewetting conditions and magnetic force were measured, and it
was shown that in the worst case, minimum 90% of the introduced cells reached to the edge of
the paper. The paper based magnetophoretic lateral flow systems can be used for cell assays.
